Method for manufacturing L-amino acids using improved strains of the enterobacteriaceae family by means of fermentation

Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae

Procédé de fabrication fermentative dýacides aminés L en utilisant des souches améliorées de la famille des enterobacteriaceae

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Mikroorganismen der Familie der Enterobacteriaceae, die ein abgeschwächtes proP-Gen enthalten, die für die Produktion geeigneten Mikroorganismen sowie Polynukleotide, die für Varianten des Transporters ProP kodieren.
Producing L-amino acids or L-amino acid containing feed additive, comprises fermenting a microorganism of the Enterobacteriaceae, where the microorganism is used in which the proP-Gen is weakened. Independent claims are included for: (1) the microorganism of the family Enterobacteriaceae, comprising a weakened proP-Gen, where the microorganism produces L-amino acids and preferably secreted into the medium; (2) the polynucleotide having an identity of at least 98, 99 or 100% with respect to the sequence according to SEQ ID NO: in 1 (a 1503 amino acid sequence fully defined in the specification); (3) a vector comprising the polynucleotide; (4) a polypedtide having an identity of at least 98, 99 or 100% with respect to the sequence according to SEQ ID NO: in 2 (a 500 amino acid sequence fully defined in the specification); (5) a recombinant microorganism comprising the polynucleotide, the vector and/or the polypeptide; and (6) identifying microorganisms of the Enterobacteriaceae family, which improves the production of sulfur-containing L-amino acids and contains the weakened proP-Gen, comprising screening the microorganisms of the Enterobacteriaceae family having the capability of producing the sulfur-containing L-amino acids to increase methionine tolerance, isolating and propagating the mutants, optionally providing nucleic acids from the mutants, optionally preparing a nucleic acid molecule using polymerase chain reaction starting from the nucleic acid and a primer pair consisting of a first primer comprising at least 15 contiguous nucleotides from position 1-1000 of the nucleotide sequence according to SEQ ID NO: 38 (a 3503 amino acid sequence fully defined in the specification) and a second primer comprising at least 15 consecutive nucleotides from position 2504-3503 of the complementary nucleotide sequence of SEQ ID NO: 38, optionally determining the nucleotide sequence of the nucleic acid molecule, and determining the encoded amino acid sequence, optionally comparing the determined the amino acid sequence of SEQ ID NO: 2, and optionally identifying the obtained proP-mutant.

Claims

Description

Topics

Download Full PDF Version (Non-Commercial Use)

Patent Citations (33)

    Publication numberPublication dateAssigneeTitle
    DE-10031999-A1April 19, 2001Degussa, Inst Innovationstransfer An DeVerfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
    EP-0472869-A2March 04, 1992Degussa AgPlasmids of corynebacterium glutamicum and derived plasmid vectors
    EP-1115152-A1July 11, 2001Hitachi Chemical Company, Ltd.Gestanztes klebeband für halbleiter, herstellungsverfahren eines leiterrahmens mit einem solchen klebeband, leiterrahmen mit klebeband und halbleiteranordnung mit einem leiterrahmen
    EP-1215605-A1June 19, 2002Yamaha CorporationSystem zum Verteilen von Inhalten
    EP-1445310-B1May 17, 2006Consortium für elektrochemische Industrie GmbHProcédé de préparation fermentative de L-méthionine
    EP-2205754-A2July 14, 2010Metabolic ExplorerAccroissement du rendement en méthionine
    EP-2326724-A1June 01, 2011Metabolic ExplorerHerstellung von methionin ohne n-acylmethionin
    EP-2479279-A1July 25, 2012Evonik Degussa GmbHVerfahren zur fermentativen Herstellung schwefelhaltiger Aminosäuren
    JP-H10229891-ASeptember 02, 1998Mitsubishi Rayon Co Ltd, 三菱レイヨン株式会社Production of malonic acid derivative
    KR-20050079338-AAugust 10, 2005씨제이 주식회사A microorganism having an inactivated iclr gene and capable of producing a l-threonine, method for manufacturing the same strain and method for producing l-threonine using the same strain
    US-4224409-ASeptember 23, 1980Ajinomoto Company, IncorporatedMethod for producing L-proline by fermentation
    US-4601893-AJuly 22, 1986Pfizer Inc.Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
    US-4822738-AApril 18, 1989Ajinomoto Co., Inc.Transducible composite plasmid
    US-5275940-AJanuary 04, 1994Kyowa Hakko Kogyo Co., Ltd.Process for producing L-tryptophan by culturing a Corynebacterium glutamicum mutant
    US-5605818-AFebruary 25, 1997Kyowa Hakko Kogyo Co., Ltd.Process for producing L-tryptophan, L-tyrosine or L-phenylalanine
    US-5770409-AJune 23, 1998Degussa AktiengsellschaftFermentative preparation of lysine with a strain of C. glutamicum
    US-5804414-ASeptember 08, 1998Ajinomoto Co., Inc.Method of amplifying genes using artificial transposons in coryneform bacteria
    US-5840551-ANovember 24, 1998Degussa AktiengesellschaftMethod of producing L-amino acids by fermentation
    US-5939307-AAugust 17, 1999The Archer-Daniels-Midland CompanyStrains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production
    US-6221636-B1April 24, 2001Ajinomoto Co., Inc.Method for producing L-lysine
    WO-0127307-A1April 19, 2001Consortium für elektrochemische Industrie GmbHProcedes pour produire de la l-cysteine ou des derives de cette derniere par fermentation
    WO-03014330-A2February 20, 2003Degussa AgBacteries coryneformes produisant des composes chimiques ii
    WO-03040373-A2May 15, 2003Degussa AgProduction of l-lysine by genetically modified corynebacterium glutamicum strains
    WO-03074719-A2September 12, 2003Degussa AgBacterie productrice d'aminoacides et procede de preparation de l-aminoacides
    WO-2005085463-A1September 15, 2005Degussa AgProcede de preparation par fermentation d'acides amines l au moyen de bacteries coryneformes de recombinaison
    WO-2006001616-A1January 05, 2006Cj CorporationMethionine producting microorganism and method of producing l-methionine using the microorganism
    WO-2007077041-A1July 12, 2007Metabolic ExplorerProcédé de synthèse de la méthionine et de ses précurseurs, l’homosérine ou la succinylhomosérine, en employant un micro-organisme exprimant de façon amplifiée la sulfate perméase
    WO-2008127240-A1October 23, 2008Cargill, Incorporated, Cj CorporationCompositions and methods of producing methionine
    WO-2009043803-A2April 09, 2009Metabolic ExplorerIncreasing methionine yield
    WO-2010020681-A1February 25, 2010Metabolic ExplorerProducing methionine without n-acyl-methionine
    WO-9202627-A1February 20, 1992Centre National De La Recherche Scientifique (Cnrs)Corynebacteria integron, method of transformation of corynebacteria by said integron, and corynebacteria obtained
    WO-9615246-A1May 23, 1996Forschungszentrum Jülich GmbHDie genexpression in coryneformen bakterien regulierende dna

NO-Patent Citations (89)

    Title
    "Handbook of Corynebacterium glutamicum"
    "Hybaid Hybridisation Guide", 1996, HYBAID LIMITED
    "Manual of Methods for General Bacteriology", 1981, AMERICAN SOCIETY FOR BACTERIOLOGY
    "Manual of Methods for General Bacteriology", AMERICAN SOCIETY FOR BACTERIOLOGY
    "The DIG System User's Guide for Filter Hybridisation", 1995, BOEHRINGER MANNHEIM
    "The DIG System Users Guide for Filter Hybridization", 1993, FIRMA BOEHRINGER MANNHEIM GMBH
    AMANN ET AL., GENE, vol. 69, 1988, pages 301 - 315
    AMANN ET AL., GENE, vol. 69, no. 2, 1988, pages 301 - 315
    AMANN; BROSIUS, GENE, vol. 40, no. 2-3, 1985, pages 183 - 190
    BARTOLOME ET AL., GENE, vol. 102, 1991, pages 75 - 78
    BERG, TYMOCZKO; STRYER: "Biochemistry, 5th ed.,", 2002, FREEMAN AND COMPANY
    BIRGE: "Bacterial and Bacteriophage Genetics, 4th ed.,", 2000, SPRINGER VERLAG
    BLATTNER ET AL., SCIENCE, vol. 277, 1997, pages 1453 - 1462
    BOYD ET AL., JOURNAL OF BACTERIOLOGY, vol. 182, 2000, pages 842 - 847
    CARRIER; KEASLING, BIOTECHNOLOGY PROGRESS, vol. 15, 1999, pages 58 - 64
    CHMIEL: "Bioprozesstechnik 1. Einführung in die Bioverfahrenstechnik", 1991, GUSTAV FISCHER VERLAG
    CHUNG ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 86, 1989, pages 2172 - 2175
    CULHAM ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 410 - 420
    CULHAM ET AL., JOURNAL OF MOLECULAR RECOGNITION, vol. 13, 2000, pages 309 - 322
    DATABASE WPI Week 200656, Derwent Publications Ltd., London, GB; AN 2006-546018, XP002697400
    DATSENKO KA; WANNER BL., PROC NATL ACAD SEI U S A., vol. 97, no. 12, 2000, pages 6640 - 5
    DONAHUE ET AL., JOURNAL OF BACTERIOLOGY, vol. 182, no. 19, 2000, pages 5624 - 5627
    EIKMANNS ET AL., GENE, vol. 102, 1991, pages 93 - 98
    ELISE R. HONDORP ET AL: "Oxidative Stress Inactivates Cobalamin-Independent Methionine Synthase (MetE) in Escherichia coli", PROTEIN ENGINEERING FOR THERAPEUTICS, PART B, vol. 290, no. 11, 1 January 2004 (2004-01-01), pages 59, XP055050137, ISSN: 0076-6879, ISBN: 978-0-12-396962-0, DOI: 10.1371/journal.pbio.0020336
    FRANCH; GERDES, CURRENT OPINION IN MICROBIOLOGY, vol. 3, 2000, pages 159 - 164
    FREEDBERG ET AL., JOURNAL OF BACTERIOLOGY, vol. 115, no. 3, 1973, pages 816 - 823
    GAIT: "Oligonukleotide synthesis: A Practical Approach", 1984, IRL PRESS
    GLASCOCK; WEICKERT, GENE, vol. 223, 1998, pages 221 - 231
    GOMES ET AL., ENZYME AND MICROBIAL TECHNOLOGY, vol. 37, 2005, pages 3 - 18
    GRANT ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 87, 1990, pages 4645 - 4649
    GREENE RC ET AL.: "Escherichia coli and Salmonella, 2. Ausgabe", 1996, pages: 542 - 560
    GROTHE ET AL., JOURNAL OF BACTERIOLOGY, vol. 166, 1986, pages 253 - 259
    GUERRERO ET AL., GENE, vol. 138, 1994, pages 35 - 41
    HAGEMANN: "Allgemeine Genetik", 1986, GUSTAV FISCHER VERLAG
    HAMILTON CM; ALDEA M; WASHBURN BK; BABITZKE P; KUSHNER SR, J BACTERIOL., vol. 171, no. 9, 1989, pages 4617 - 4622
    HAMILTON ET AL., JOURNAL OF BACTERIOLOGY, vol. 174, 1989, pages 4617 - 4622
    HERMANN ET AL., ELECTOPHORESIS, vol. 22, 2001, pages 1712 - 23
    HERMANN ET AL., ELECTROPHORESIS, vol. 22, 2001, pages 1712.23
    HORTON: "Gene Splicing by Overlap Extension", MOLECULAR BIOTECHNOLOGY, vol. 3, 1995, pages 93 - 98
    INNIS ET AL.: "PCR Protocols. A guide to methods and applications", 1990, ACADEMIC PRESS
    J.H. MILLER: "A Short Course in Bacterial Genetics", 1992, COLD SPRING HARBOR LABORATORY PRESS
    JENSEN; HAMMER, BIOTECHNOLOGY AND BIOENGINEERING, vol. 58, 1998, pages 191 - 195
    KATOH, GENOME INFORMATION, vol. 16, no. 1, 2005, pages 22 - 33
    KAWANO ET AL., NUCLEIC ACIDS RESEARCH, vol. 33, no. 19, 2005, pages 6268 - 6276
    KNIPPERS: "Molekulare Genetik, 6. Auflage,", 1995, GEORG THIEME VERLAG
    KUNTE ET AL., JOURNAL OF BACTERIOLOGY, vol. 181, 1999, pages 1537 - 43
    LABARRE ET AL., JOURNAL OF BACTERIOLOGY, vol. 175, 1993, pages 1001 - 1007
    LERNER, C.G.; INOUYE, M., NUCL. ACIDS RES., vol. 18, 1990, pages 4631
    LIEBL ET AL., INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, vol. 41, 1991, pages 255 - 260
    LIND ROTH ET AL., ANALYTICAL CHEMISTRY, vol. 51, 1979, pages 1167 - 1174
    LINK ET AL., JOURNAL OF BACTERIOLOGY, vol. 179, pages 6228 - 6237
    LINK, A.J.; PHILLIPS, D.; CHURCH, G.M., J. BACTERIOLOGY, vol. 179, 1997, pages 6228 - 6237
    LOHAUS ET AL., BIOSPEKTRUM, vol. 5, 1998, pages 32 - 39
    LOHAUS; MEYER, BIOSPEKTRUM, vol. 5, 1989, pages 32 - 39
    LOTTSPEICH, ANGEWANDTE CHEMIE, vol. 111, 1999, pages 2630 - 2647
    MALUMBRES ET AL., GENE, vol. 134, 1993, pages 15 - 24
    MARTIN ET AL., BIO/TECHNOLOGY, vol. 5, 1987, pages 137 - 146
    MARTINEZ-MORALES ET AL., JOURNAL OF BACTERIOLOGY, 1999, pages 7143 - 7148
    MELLIES J ET AL: "TWO DIFFERENT ESCHERICHIA COLI PROP PROMOTERS RESPOND TO OSMOTIC AND GROWTH PHASE SIGNALS", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 177, no. 1, 1 January 1995 (1995-01-01), pages 144 - 151, XP000578578, ISSN: 0021-9193
    MILLER: "A Short Course in Bacterial Genetics, A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria", 1992, COLD SPRING HARBOR LABORATORY PRESS
    NAKAYAMA: "Overproduction of Microbial Products", 1982, ACADEMIC PRESS, article "Breeding of Amino Acid Producing Microorganisms"
    NATURE GENETICS, vol. 20, 1998, pages 123 - 128
    NEWTON; GRAHAM: "PCR", 1994, SPEKTRUM AKADEMISCHER VERLAG
    OLD I G ET AL: "Regulation of methionine biosynthesis in the enterobacteriaceae", PROGRESS IN BIOPHYSICS AND MOLECULAR BIOLOGY, PERGAMON PRESS, OXFORD, GB, vol. 56, no. 3, 1 January 1991 (1991-01-01), pages 145 - 185, XP025215159, ISSN: 0079-6107, [retrieved on 19910101], DOI: 10.1016/0079-6107(91)90012-H
    PROC NATL ACAD SEI U S A., vol. 97, no. 12, 2000, pages 6640 - 6645
    QIU; GOODMAN, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
    RACHER ET AL., BIOCHEMISTRY, vol. 38, 1999, pages 1676 - 1684
    RACHER ET AL., BIOCHEMISTRY, vol. 40, 2001, pages 7324 - 7333
    RAY ET AL., JOURNAL OF BACTERIOLOGY, vol. 182, no. 8, 2000, pages 2277 - 2284
    REINSCHEID ET AL., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 60, 1994, pages 126 - 132
    SAMBROOK ET AL.: "Molecular Cloning - A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
    SAMBROOK ET AL.: "Molecular Cloning: a laboratory manual, 2nd Ed.", 1989, COLD SPRING HARBOR LABORATORY PRESS
    SAMBROOK ET AL.: "Molecular Cloning: a laboratory manual, 2nd Ed.", COLD SPRING HARBOR LABORATORY PRESS
    SAMBROOK ET AL.: "Molekular Cloning, A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR LABORATORY PRESS
    SANGER ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE USA, vol. 74, no. 12, 1977, pages 5463 - 5467
    SCHWARZER; PÜHLER, BIO/TECHNOLOGY, vol. 9, 1991, pages 84 - 87
    SPACKMAN ET AL., ANALYTICAL CHEMISTRY, vol. 30, 1958, pages 1190
    STORHAS: "Bioreaktoren und periphere Einrichtungen", 1994, VIEWEG VERLAG
    T. A. BROWN: "Gentechnologie für Einsteiger", 1993, SPEKTRUM AKADEMISCHER VERLAG
    THOMPSON ET AL., NUCLEIC ACIDS RESEARCH, vol. 22, 1994, pages 4637 - 4680
    TSUCHIYA; MORINAGA, BIO/TECHNOLOGY, vol. 6, 1988, pages 428 - 430
    VOCKE; BASTIA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 80, no. 21, 1983, pages 6557 - 6561
    WENTE; SCHACHMANN, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, 1991, pages 20833 - 20839
    WILSON ET AL., JOURNAL OF BACTERIOLOGY, vol. 183, 2001, pages 2151 - 2155
    WINNACKER: "Gene und Klone", 1990, VCH VERLAGSGESELLSCHAFT
    WOOD, METHODS IN ENZYMOLOGY, vol. 428, 2007, pages 77 - 107
    WOOD, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 63, 1999, pages 230 - 262
    YANO ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, 1998, pages 5511 - 5515
    YOSHIHIRO USUDA; OSAMU KURAHASHI, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 6, 2005, pages 3228 - 3234

Cited By (0)

    Publication numberPublication dateAssigneeTitle